[Comparison of solid phase and liquid-liquid extraction of diazepam and nitrazepam]

We have developed a new extraction technique for nitrazepam and diazepam using Samplex C-18 column. The blood-extractions containing diazepam and nitrazepam were diluted with acetonitrile-water 7:3 eluent. Diazepam and nitrazepam were isolated using liquid-liquid extraction technique. The blood-extracts were diluted with chloroform-ethanol 1:1 and they were evaluated by using OPLC method. Evaluation was done by TLC-scanner (Shimadzu). The efficiency and sensitivity of the methods have been compared.