Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Practical time-gated luminescence flow cytometry. I: concepts.

Dayong Jin1, Russell Connally, James Piper

  • 1Centre for Lasers and Applications, Division of Information and Communication Sciences, Macquarie University, NSW, Australia. jin@ics.mq.edu.au

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|September 18, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hardware-Attentive Programmable Fourier Ptychography Enables Task-Adaptive Label-Free Virtual Staining.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Correction to "Thiolate DNAzymes on Gold Nanoparticles for Isothermal Amplification and Detection of Mesothelioma-derived Exosomal PD-L1 mRNA".

Analytical chemistry·2026
Same author

Binocular vision fusion enhanced 3D NIR-II <i>in vivo</i> imaging of bone and vessel networks.

Fundamental research·2026
Same author

Beads-on-a-Tip testing for ultrasensitive antigen detection across a large dynamic range.

Smart molecules : open access·2026
Same author

Lattice and ligand engineering for hierarchical heterogeneous nanocrystals.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

White matter microstructure and cognitive decline in subacute ischemic stroke: Insights from free water imaging and glymphatic dysfunction.

Brain research bulletin·2026
Same journal

The 1st Mediterranean Meeting on Flow Cytometry: Forging New Collaborations Across the Mediterranean and Beyond.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same journal

Publication Guidelines for Optimized Multiparameter Immunolabeling Panels (OMIPs).

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same journal

A Modular High-Parameter Flow Cytometry Framework: Pre-Analytical Optimization and Validation for Clinical Research.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same journal

Quantitative Detection of Entotic Cell-In-Cell Structures Using Deformable Segmentation and Deep Learning.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same journal

Comparison of Tissue Preparations to Identify and Phenotype T Cells in Human Colorectal Tumor Tissue.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same journal

Refractive Index-Correlated Pseudocoloring for Adaptive Color Fusion in Holotomographic Cytology.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
See all related articles

Time-gated luminescence (TGL) flow cytometry enables high-speed cell analysis by suppressing background noise. New UV LED technology and practical models achieve 100% detection efficiency for labeled microorganisms.

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Optical Physics

Background:

  • Time-gated detection effectively reduces autofluorescence and light scatter in luminescence-based assays.
  • Applying time-gated detection to flow cytometry presents challenges in achieving high throughput for rapidly flowing samples.
  • Existing methods struggle to balance high cell analysis rates with efficient detection in flow cytometry.

Purpose of the Study:

  • To develop practical methods for high-throughput cell analysis using time-gated luminescence (TGL) flow cytometry.
  • To investigate the feasibility of new-generation UV LEDs as excitation sources for TGL flow cytometry.
  • To analyze the theoretical requirements and practical implementation of TGL in a continuous flow system.

Main Methods:

  • Investigated spatial effects of long-lived luminescence in fast-flowing samples.

Related Experiment Videos

  • Theoretically analyzed excitation and detection parameters for TGL flow cytometry.
  • Developed and evaluated two models: a triggered mode and a continuous flow-section mode.
  • Modeled a system using UV LED excitation for europium dye-labeled targets.

    • Main Results:

    • New-generation UV LEDs are suitable excitation sources for TGL flow cytometry.
    • The triggered model operates at low repetition rates, while the flow-section model allows high cell arrival rates.
    • Demonstrated feasible detection of europium dye-labeled beads with a signal-to-background ratio up to 11:1.
    • Achieved 100% detection efficiency at high cell analysis rates.

    Conclusions:

    • Time-gated luminescence flow cytometry, particularly with UV LED excitation, offers a practical solution for high-throughput cell analysis.
    • The developed flow-section model enables significantly higher cell analysis rates compared to triggered systems.
    • This approach effectively suppresses background noise, enhancing signal detection for luminescence-labeled targets.