Related Experiment Video
Updated: Jul 11, 2026

Continuous Flow Chemistry: Reaction of Diphenyldiazomethane with p-Nitrobenzoic Acid
Published on: November 15, 2017
Flow-injection determination of acetone with diazotized anthranilic acid through a fluorescent reaction intermediate
C García de María1, K B Hueso Domínguez, N Martín Garrido
1Dpto. de Química Analítica, Nutrición y Bromatología, University of Salamanca, Plaza de la Merced s/n, E-37008 Salamanca, Spain.
Abstract:
Acetone and diazotized anthranilic acid react in alkaline solution, giving a fluorescent intermediate that can be measured at excitation and emission wavelengths of 305 and 395 nm, respectively. Based on this, a fluorimetric flow-injection method is proposed for the determination of acetone in aqueous solution. Under the proposed conditions, acetone can be detected at concentrations higher than 8 x 10(-7)M, with a linear application range from 1 x 10(-6) to 2 x 10(-4)M and an R.S.D. of 2.7% (1.0 x 10(-5)M, n=10). A sampling frequency of 24h(-1) is achieved. Some potentially interfering species are investigated.
Related Concept Videos
Carboxylic Acids to Methylesters: Alkylation using Diazomethane
1° Amines to Diazonium or Aryldiazonium Salts: Diazotization with NaNO2 Mechanism
Aryldiazonium Salts to Azo Dyes: Diazo Coupling
Diazonium Group Substitution: –OH and –H
Nucleophilic Aromatic Substitution of Aryldiazonium Salts: Aromatic SN1
In the Sandmeyer reaction, for example, the diazonio group is replaced by a chloro, bromo, or cyano...
Flame Photometry: Lab

