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Updated: Jul 11, 2026
Protective Efficacy and Pulmonary Immune Response Following Subcutaneous and Intranasal BCG Administration in Mice
Published on: September 19, 2016
[Immune response induced by Mycobacterium tuberculosis Rv2450 protein in mice]
Ying Xue1, Yin-Lan Bai, Hui Gao
1Department of Microbiology, Fourth Military Medical University, Xi'an, China. x.ying@yeah.net
Aim:
To evaluate humoral and cellular immune response induced by Rv2450 protein.
Methods:
Rv2450 protein was transferred to membrane and used to immunize C57BL/6 mice three times at 2 week interval. The spleen lymphocytes of the immunized mice were separated and the stimulation index (SI) was measured by MTT colorimetry and the levels of secreted IFN-gamma, IL-10 and IL-12 upon antigen-specific stimulation was detected by ELISA.
Results:
The titer of specific antibody in sera of the immunized C57BL/6 mice was 1:3 200. The SI of Rv2450 protein immunized group (3.76 +/- 0.19) was significantly higher than that of saline immunized group (0.89+/-0.17). The IFN-gamma, IL-10, IL-12 levels in culture supernatant of spleen lymphocytes from the immunized mice was (1 740 +/- 19) ng/L, (678 +/- 15) ng/L and (469 +/- 13) ng/L respectively, significantly different from that of saline-immunized group.
Conclusion:
Rv2450 protein can be used as a candidate for the new TB vaccine.
