Related Experiment Video
Updated: Jul 11, 2026

ARL Spectral Fitting as an Application to Augment Spectral Data via Franck-Condon Lineshape Analysis and Color Analysis
Published on: August 19, 2021
Concentration dependence in the spectra of polycyclic aromatic hydrocarbon mixtures by front-surface fluorescence
Joseph F Sinski1, Jeffrey Exner
1Department of Chemistry, Bellarmine University, 2001 Newberg Road, Louisville, KY 40205, USA. jsinski@bellarmine.edu
Abstract:
Polycyclic aromatic hydrocarbons (PAHs) are a challenge for fluorescence analysis. Inner-filter quenching effects and energy transfers of PAHs in petroleum differ with concentration, leading to characteristically different fluorescence. At high concentrations of single PAH molecules and petroleum mixtures the classic rectangular quartz cell presents a particular challenge due to inner filter effects from right-angle illumination. While quite a few instrumental and mathematical corrections for these effects are reported throughout the literature, for researchers with standard instrumental sample compartments a triangular fluorometer cell can be used to emulate front-surface fluorescence analysis. This work presents concentration study results from the single PAH molecules fluoranthene and azulene dissolved in spectral-grade cyclohexane and also seeks to correct an aspect of our past work on the fluorescence of petroleum mixtures.
More Related Videos
Related Concept Videos
Variables Affecting Phosphorescence and Fluorescence
Flame Photometry: Lab
Atomic Fluorescence Spectroscopy
UV–Vis Spectroscopy of Conjugated Systems
One of the factors influencing λmax is the extent of conjugation in the...
Photoluminescence: Applications
Mass Spectrometry: Aromatic Compound Fragmentation

