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LHR splicing variants and gene expression in the marmoset monkey
Caroline Michel1, Jörg Gromoll, Ramesh Chandolia
1Institute of Reproductive Medicine of the University, Domagkstrasse 11, D-48149 Muenster, Germany.
Molecular and Cellular Endocrinology
|October 5, 2007
Summary
Marmoset monkeys primarily use LHR type II, with consistent splicing patterns and expression levels throughout puberty. This suggests LHR
Area of Science:
- Reproductive endocrinology
- Molecular biology
- Primate research
Background:
- The luteinizing hormone receptor (LHR) plays a crucial role in reproductive functions.
- LHR type II, lacking exon 10, is identified as the native receptor type in marmoset monkeys.
- Understanding LHR splicing and expression is vital for comprehending reproductive maturation in primates.
Purpose of the Study:
- To characterize the LHR splicing pattern in marmoset testes and adrenals during puberty.
- To analyze LHR splicing and mRNA expression in pre- and postpubertal ovaries.
- To investigate age-related changes in LHR expression and its relationship with testosterone levels.
Main Methods:
- Analysis of LHR splicing variants in marmoset reproductive and adrenal tissues.
- Quantification of LHR mRNA expression in the testis.
- Assessment of the serum testosterone/LHR ratio during pubertal maturation.
Main Results:
- Eleven LHR splicing variants were detected, generated through exon skipping and cryptic splice site usage, with similar expression levels.
- No specific LHR splicing variant preference was observed during pubertal maturation in either sex.
- Testicular and adrenal LHR mRNA expression remained constant with age, but the serum testosterone/LHR ratio increased significantly during puberty.
Conclusions:
- Marmoset LHR splicing exhibits a homogenous pattern, indicating a conserved mechanism.
- The primary function of LHR is established in the testis, with peak efficiency during pubertal maturation.
- Constant LHR mRNA levels alongside increased testosterone secretion suggest a finely tuned regulatory process during primate reproductive development.
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