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Systematic Analysis of In Vitro Cell Rolling Using a Multi-well Plate Microfluidic System
Published on: October 16, 2013
Automated live cell screening system based on a 24-well-microplate with integrated micro fluidics
V Lob1, T Geisler, M Brischwein
1Heinz Nixdorf-Chair for Medical Electronics at the Technical University of Munich, Theresienstr. 90, Gebäude N3, 80333, Munich, Germany.
Medical & Biological Engineering & Computing
|October 6, 2007
Summary
This study introduces a high-content system for real-time monitoring of living cells. The platform tracks metabolic and morphological changes, offering dynamic insights crucial for drug screening and diagnostics.
Area of Science:
- Cell Biology
- Pharmacology
- Biomedical Engineering
Background:
- Functional assays using living cells are increasingly vital in drug screening and diagnostics.
- Conventional endpoint measurements fail to capture dynamic cellular processes.
- Monitoring extracellular parameters over time offers deeper insights into cellular behavior.
Purpose of the Study:
- To describe a high-content system for real-time, multi-parametric monitoring of living cells.
- To address the need for dynamic data in cell-based assays.
- To integrate sensor readout, imaging, and probe handling in a single platform.
Main Methods:
- Developed a high-content system for parallel monitoring of 24 cell cultures.
- Measured extracellular parameters including acidification rates and oxygen consumption.
- Integrated automated imaging, probe handling, and a life-maintenance system.
Main Results:
- The system provides real-time data on metabolic and morphological cell alterations.
- Enabled parallel monitoring of acidification, oxygen consumption, and adhesion forces.
- Demonstrated a platform for comprehensive, dynamic cell analysis.
Conclusions:
- The developed system facilitates advanced, real-time analysis of cellular responses.
- It supports high-content screening assays by providing multi-parametric, dynamic data.
- The integrated platform enhances the study of cellular processes in pharmacology and diagnostics.
