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Related Concept Videos

Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...

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Related Experiment Video

Updated: Jul 10, 2026

Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry
09:20

Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry

Published on: April 11, 2022

[Destroy this (RNA) message after reading it!].

Sylvie Camier1, Bertrand Séraphin

  • 1Equipe Labellisée la ligue, Centre de Génétique Moléculaire, CNRS UPR2167, associée à l'Université Pierre et Marie Curie, Avenue de la terrasse, 91198 Gif-sur-Yvette, France.

Medecine Sciences : M/S
|October 17, 2007
PubMed
Summary
This summary is machine-generated.

mRNA decay is a crucial gene expression regulator, not just mRNA destruction. It involves quality control for aberrant transcripts and regulates mRNA stability via specific factors.

More Related Videos

Depletion of Ribosomal RNA for Mosquito Gut Metagenomic RNA-seq
06:21

Depletion of Ribosomal RNA for Mosquito Gut Metagenomic RNA-seq

Published on: April 7, 2013

Related Experiment Videos

Last Updated: Jul 10, 2026

Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry
09:20

Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry

Published on: April 11, 2022

Depletion of Ribosomal RNA for Mosquito Gut Metagenomic RNA-seq
06:21

Depletion of Ribosomal RNA for Mosquito Gut Metagenomic RNA-seq

Published on: April 7, 2013

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • RNA Biology

Context:

  • mRNA decay was traditionally viewed as a simple degradation process.
  • Recent research highlights mRNA decay's critical role in gene expression regulation.
  • Understanding the cellular localization of mRNA decay reactions is essential.

Purpose:

  • To review the biochemical reactions involved in mRNA decay.
  • To explore the role of mRNA decay in cellular quality control mechanisms.
  • To describe how mRNA decay regulates gene expression through stability control.

Summary:

  • This review details mRNA decay pathways, including nonsense-mediated decay, non-stop decay, and no-go decay, which eliminate aberrant mRNAs.
  • It also covers the regulation of mRNA stability by specific targeting factors like proteins and small non-coding RNAs.
  • The cellular locations of these decay processes are also discussed.

Impact:

  • Revises the traditional view of mRNA decay from simple destruction to a key regulatory mechanism.
  • Provides insights into mRNA quality control systems essential for cellular function.
  • Highlights the complex roles of mRNA decay in maintaining cellular homeostasis and gene expression fidelity.