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Related Experiment Videos

Strategies to induce nuclear reprogramming.

S Eminli1, R Jaenisch, K Hochedlinger

  • 1Center for Regenerative Medicine and Cancer Center, Massachusetts General Hospital, Harvard Medical School and Harvard Stem Cell Institute, 185 Cambridge Street, 02114 Boston, USA.

Ernst Schering Foundation Symposium Proceedings
|October 18, 2007
PubMed
Summary
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Mammalian cloning shows oocytes reprogram adult cells to a pluripotent state. Future work aims to stably convert adult cells into embryonic stem cells using defined factors for disease treatment.

Area of Science:

  • Cell biology
  • Developmental biology
  • Stem cell research

Background:

  • Mammalian cloning demonstrates oocyte reprogramming of adult nuclei to pluripotency.
  • Cellular reprogramming can be induced via germ line cell explantation or fusion with embryonic cells.

Purpose of the Study:

  • To explore stable conversion of differentiated cells into embryonic stem (ES) cells.
  • To investigate the potential of transient gene expression for cellular reprogramming.
  • To assess the feasibility of generating patient-specific stem cells for disease treatment.

Main Methods:

  • Reprogramming of differentiated cells using oocyte factors.
  • Explantation of germ line cells for reprogramming.
  • Fusion of differentiated cells with embryonic cells.

Related Experiment Videos

  • Transient expression of defined genes in adult cells.

    • Main Results:

    • Oocytes can reprogram differentiated nuclei to a pluripotent embryonic state.
    • Exposure of adult cells to defined factors induces a pluripotent-like state.
    • This approach offers an alternative to nuclear transfer for generating patient-specific stem cells.

    Conclusions:

    • Stable conversion of differentiated cells to ES cells is a future challenge.
    • Transient expression of defined factors shows promise for inducing pluripotency.
    • Patient-specific stem cells generated through this method could advance the study and treatment of degenerative diseases.