[A technique for investigating the component of cantharidin blisters by flow cytometry]

Cantharidimide cause blister. The effect of blister on immunoregulation was investigated.

Cantharidimide was placed on the skin, 48h later, the blister was analyzed by flow cytometry.

The blister contained 1 x 10(6) - 1 x 10(7) cells per ml, most of which were neutrophils, macrophages, dendritic cells (DC), and IL-12 secreted by Thl cells.

There are high concent of DC in the blister, which is differential and induce the secretion of Th1, the activation of T cell. The blister modulate the biological response of patients and is helpful for treatment with infective disease.