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NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
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Visualization of Neutrophil Extracellular Traps in Mesenteric Venules After Mesenteric Ischemia-Reperfusion Injury via Intravital Microscopy
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Postshock mesenteric lymph induces endothelial NF-kappaB activation.

Sagar S Damle1, Ernest E Moore, Trevor L Nydam

  • 1University of Colorado at Denver, Health Sciences Center, Denver, Colorado 80204, USA.

The Journal of Surgical Research
|October 24, 2007
PubMed
Summary

Posthemorrhagic shock mesenteric lymph (PSML) causes lung injury by degrading IkappaB and activating nuclear factor-kappa B (NF-kappaB). Targeting these pathways may prevent organ damage after shock.

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Visualization of Neutrophil Extracellular Traps in Mesenteric Venules After Mesenteric Ischemia-Reperfusion Injury via Intravital Microscopy
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Murine Model of Intestinal Ischemia-reperfusion Injury
07:07

Murine Model of Intestinal Ischemia-reperfusion Injury

Published on: May 11, 2016

Area of Science:

  • Cellular and Molecular Biology
  • Immunology
  • Pathophysiology

Background:

  • Posthemorrhagic shock mesenteric lymph (PSML) activates pulmonary endothelial cells, leading to lung injury.
  • Nuclear factor-kappa B (NF-kappaB) activation is a primary mediator of these effects.
  • Degradation of inhibitor of kappa B (IkappaB) is crucial for NF-kappaB activation.

Purpose of the Study:

  • To investigate if PSML causes IkappaB degradation.
  • To determine if PSML leads to NF-kappaB phosphorylation and nuclear translocation.

Main Methods:

  • Mesenteric lymph was collected from rats post-shock.
  • Human pulmonary endothelial cells were treated with lymph or tumor necrosis factor.
  • Immunoblots and immunohistochemistry were used to analyze IkappaB and NF-kappaB (p65) levels and localization.

Main Results:

  • A decrease in total IkappaB was observed with increasing PSML exposure.
  • Phosphorylation of NF-kappaB's p65 component increased with PSML.
  • Significant increase in NF-kappaB nuclear translocation was noted with PSML.

Conclusions:

  • Postshock mesenteric lymph bioactivity involves IkappaB degradation pathways.
  • These pathways represent potential therapeutic targets to prevent organ injury following hemorrhagic shock.