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Updated: Aug 15, 2026

Fizzy Extraction of Volatile Organic Compounds Combined with Atmospheric Pressure Chemical Ionization Quadrupole Mass Spectrometry
Published on: July 14, 2017
Application of microcolumn liquid chromatography-continuous-flow fast atom bombardment mass spectrometry in
R W Reiser1, A C Barefoot, R F Dietrich
1E. I. du Pont de Nemours and Company, Agricultural products Department, Wilmington, DE 19880-0402.
Abstract:
The use of 0.25-mm I.D. packed capillary liquid chromatography columns coupled with continuous-flow fast atom bombardment (FAB) mass spectrometry has proven to be a very valuable technique, especially for the identification of unknown sulfonylurea herbicide metabolites. Several new and unusual heterocycle ring-opened metabolites and hydrolysis products were identified, and metabolic pathways were proposed. Typical column flow-rates are 1-2 microliters/min, which allows direct coupling with no sample splitting. This is important in our metabolite identification work, since we are usually sample-limited. Techniques for increasing injection volume to allow analyses of dilute solutions and the use of polymeric packing for separation of polar metabolites are discussed. The FAB mass spectra usually provide unequivocal molecular weights and structurally useful fragments ions, which often allows structure assignments on exceedingly small quantities of isolated metabolites.
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