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Updated: Jul 10, 2026

Physiological Preparation of Hair Cells from the Sacculus of the American Bullfrog (Rana catesbeiana)
Published on: March 17, 2017
In situ binding assay to detect Myosin-1c interactions with hair-cell proteins
Kelli R Phillips1, Janet L Cyr
1Sensory Neuroscience Research Center, Department of Biochemistry and Molecular Pharmacology, West Virginia University School of Medicine, Morgantown, WV, USA.
Abstract:
Myosin-1c is an unconventional myosin involved in hair-cell mechanotransduction, a process that underlies our senses of hearing and balance. To study the interaction of myosin-1c with other components of the hair-cell transduction complex, we have developed an in situ binding assay that permits visualization of myosin-1c binding to hair-cell proteins. In this chapter we describe in detail the methods needed for the expression and purification of recombinant myosin-1c fragments and their use in the in situ binding assay.

