Related Experiment Video
Updated: Jul 10, 2026
Longitudinal Intravital Imaging Through Clear Silicone Windows
Published on: January 5, 2022
[Histological and morphometric analysis of the raw areas treated with a silicone membrane: study in rats]
Eduardo Carlos da Silveira Mendes1, Fausto Viterbo, Celina Santaella Rosa
1Faculdade de Medicina de Botucatu, Universidade Estadual Paulista Julio Mesquita Filho, UNESP.
Background:
This study evaluated the effects of a silicone membrane on the treatment of the raw flesh area in wounds.
Methods:
The experimental study was carried out with 30 male Wistar rats divided into three groups: in the GG group, the raw area was treated with gauze, in the GH group with homograft, and in the GS group with a silicone membrane. Animals were sacrificed at fourteen days of postoperative. The studied attributes were body mass variation, histological study quantified by morphometric analysis evaluating the number of neovessels, fibroblasts, collagen fibers, leucocytes, monocytes; and using a micrometric ruler measurement of the raw area's thickness. Data were then submitted to statistical analysis.
Results:
There was no significant difference between the animals' mass (p=0.0685). Predominance of neovessels (p<0.01), fibroblasts (p<0.001) and thickness of the raw flesh were observed in G S animals. There was predominance of leucocytes (p<0.021) and monocytes (p<0.0001) in G H animals. Also, no significant difference between the groups as for collagen fibers (p=0.0536) was observed.
Conclusion:
The silicone membrane promoted granulation tissue with a large number of neovessels, fibroblasts and greater thickness.
