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Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
Centrifugation01:05

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...

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Related Experiment Video

Updated: Jul 10, 2026

Primary Clarification of CHO Harvested Cell Culture Fluid using an Acoustic Separator
07:06

Primary Clarification of CHO Harvested Cell Culture Fluid using an Acoustic Separator

Published on: May 14, 2020

Continuous blood cell separation by hydrophoretic filtration.

Sungyoung Choi1, Seungjeong Song, Chulhee Choi

  • 1Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 335 Gwahangno, Yuseong-gu, Daejeon, 305-701, Republic of Korea.

Lab on a Chip
|October 26, 2007
PubMed
Summary

A new microfluidic device uses hydrophoretic filtration for continuous blood cell separation. This method efficiently isolates white blood cells from red blood cells, offering a power-free solution for lab-on-a-chip devices.

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Isolating Human Peripheral Blood Mononuclear Cells from Buffy Coats via High Throughput Immunomagnetic Bead Separation

Published on: July 19, 2024

Area of Science:

  • Biomedical Engineering
  • Microfluidics
  • Cell Separation Technology

Background:

  • Continuous blood cell separation is crucial for diagnostics and research.
  • Existing methods can be time-consuming or require complex equipment.
  • Microfluidic devices offer miniaturized and efficient solutions for biological sample processing.

Purpose of the Study:

  • To develop a novel hydrophoretic method for continuous blood cell separation.
  • To design a microfluidic device capable of size-based particle separation.
  • To evaluate the device's efficiency in isolating white blood cells (WBCs) from red blood cells (RBCs).

Main Methods:

  • Utilized a microfluidic device with slanted and filtration obstacles.
  • Employed hydrophoresis to focus particles towards a sidewall.
  • Engineered filtration obstacles with specific pore sizes for size-based particle sorting.

Main Results:

  • Achieved complete separation of 9 and 12 micrometer polystyrene microbeads with high resolution (6.2), a 6.4-fold improvement over previous methods.
  • Isolated white blood cells (WBCs) from red blood cells (RBCs) with 210-fold enrichment.
  • Demonstrated efficient separation within a short filtration time of approximately 0.3 seconds.

Conclusions:

  • The proposed hydrophoretic filtration method enables efficient, size-based binary separation of biological particles.
  • The microfluidic device is suitable for isolating specific cell populations, such as WBCs from blood.
  • This technology holds potential for developing power-free, integrated cell sorters for disposable lab-on-a-chip applications.