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Related Experiment Video

Updated: Jul 10, 2026

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)

Published on: June 28, 2017

Supercontinuum dynamically visualizes a dividing single cell.

Hideaki Kano¹, Hiro-o Hamaguchi

¹Department of Chemistry, School of Science, The University of Tokyo, Hongo 7-3-1, Bunkyo, Tokyo 113-0033, Japan.

Analytical Chemistry

|October 31, 2007

Summary

Researchers developed a new direct cell imaging method using Coherent Anti-Stokes Raman Scattering (CARS) spectroscopy. This technique visualizes live cell division dynamics without fluorescent probes, offering molecular-level insights.

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Area of Science:

Cell biology
Spectroscopy
Microscopy

Background:

Organelle dynamics during cell division are crucial for understanding life at the molecular level.
Current fluorescence microscopy methods require staining, which can alter cellular conditions.
Direct visualization of live cell processes is needed to overcome limitations of staining-based techniques.

Purpose of the Study:

To develop a novel, direct visualization method for living cell processes.
To enable high-resolution imaging of cell division dynamics without invasive staining.
To advance molecular-level understanding of cellular behaviors.

Main Methods:

Utilized Coherent Anti-Stokes Raman Scattering (CARS) spectroscopy for direct cellular imaging.
Employed a supercontinuum light source generated from photonic crystal fiber for ultrabroadband multiplex CARS.
Developed multiple nonlinear spectral imaging by simultaneously measuring CARS and two-photon excited fluorescence (TPEF) spectra.

Main Results:

Successfully traced the entire cell division process, including septum formation and daughter cell splitting.
Visualized dynamic distribution changes of lipid membrane-bound organelles in real-time.
Achieved high-speed, three-dimensional image reconstruction of living cells with high molecular specificity.

Conclusions:

Multiplex CARS spectroscopy with a supercontinuum light source provides a powerful, non-invasive tool for studying live cell dynamics.
This technique overcomes the limitations of traditional staining methods in cell biology research.
Enables unprecedented insights into molecular-level mechanisms of cell division and organelle behavior.