Related Experiment Video
Updated: Jul 10, 2026

Flow Cytometric Characterization of Murine B Cell Development
Published on: January 22, 2021
Phenotypic analysis of B-cells and plasma cells
Henrik E Mei1, Taketoshi Yoshida, Gwendolin Muehlinghaus
1German Rheumatism Research Centre, Berlin, Germany.
Abstract:
B-cells and antibody-secreting plasma cells are key players in protective immunity, but also in autoimmune disease. To understand their various functions in the initiation and maintenance of autoimmune pathology, a detailed dissection of their functional diversity is mandatory. This requires a detailed phenotypic classification of the diversity of B-cells. Here, technologies of immunocytometry and ELISpot are described in detail, and their value for phenotypic characterization of cells of the B lineage, as well as for preparative cell sorting, to further characterize them functionally and on the molecular level are described.

