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Updated: Jul 10, 2026
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Flow Cytometric Characterization of Murine B Cell Development
Published on: January 22, 2021
Phenotypic analysis of B-cells and plasma cells
Henrik E Mei1, Taketoshi Yoshida, Gwendolin Muehlinghaus
1German Rheumatism Research Centre, Berlin, Germany.
Methods in Molecular Medicine
|November 7, 2007
Summary
Understanding B-cell diversity is crucial for both immunity and autoimmune disease. This study details immunocytometry and ELISpot methods for phenotypic B-cell classification and functional analysis.
Area of Science:
- Immunology
- Cell Biology
Background:
- B-cells and plasma cells are vital for immunity but also implicated in autoimmune diseases.
- Understanding their diverse roles in autoimmune pathology requires detailed functional dissection.
Purpose of the Study:
- To provide a detailed phenotypic classification of B-cell diversity.
- To explore the utility of immunocytometry and ELISpot for characterizing B-cell lineage cells.
Main Methods:
- Detailed description of immunocytometry techniques.
- Detailed description of ELISpot assays.
- Application of these methods for phenotypic characterization and cell sorting.
Main Results:
- Established detailed phenotypic classification of B-cell diversity.
- Demonstrated the value of immunocytometry and ELISpot for B-cell characterization.
- Facilitated functional and molecular characterization through preparative cell sorting.
Conclusions:
- Phenotypic classification of B-cell diversity is essential for understanding their roles in health and disease.
- Immunocytometry and ELISpot are powerful tools for B-cell analysis.
- These methods enable further functional and molecular studies of B-cell subsets.
