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Quantification of Cytosolic vs. Vacuolar Salmonella in Primary Macrophages by Differential Permeabilization
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Membrane dynamics and spatial distribution of Salmonella-containing vacuoles

Amy E Ramsden1, David W Holden, Luís J Mota

  • 1Centre for Molecular Microbiology and Infection, Imperial College London, Armstrong Road, London, UK.

Trends in Microbiology
|November 7, 2007
PubMed

Insights

Salmonella enterica bacteria hijack host cell machinery to replicate inside Salmonella-containing vacuoles. Type III secretion effectors manipulate microtubule motors and vesicular trafficking for intracellular survival.

Area of Science:

  • Microbiology
  • Cell Biology
  • Infectious Diseases

Background:

  • Salmonella enterica are facultative intracellular bacteria causing intestinal and systemic diseases.
  • Bacterial replication occurs within a membrane-bound compartment, the Salmonella-containing vacuole (SCV).
  • SCV dynamics and intracellular positioning are crucial for bacterial survival and depend on host cell vesicular compartments.

Purpose of the Study:

  • To discuss current models of Salmonella type III secretion effector proteins.
  • To explain how these effectors influence SCV dynamics and intracellular positioning.
  • To elucidate the hijacking of host cell microtubule motors and Golgi interactions.

Main Methods:

  • Review of recent studies on Salmonella type III secretion effector proteins.
  • Analysis of effector interactions with host cell vesicular compartments.
  • Discussion of effector roles in manipulating microtubule motors (kinesins and dynein).

Main Results:

  • Type III secretion effectors regulate SCV membrane dynamics and intracellular positioning.
  • Effectors hijack host cell microtubule motors, including kinesins and dynein.
  • Effectors appear to interact with the host cell's Golgi complex.

Conclusions:

  • Salmonella employs sophisticated strategies involving type III secretion effectors to control its intracellular niche.
  • Understanding these effector functions provides insights into host-pathogen interactions and bacterial pathogenesis.
  • These mechanisms highlight the complex interplay between bacterial virulence factors and host cell processes.

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