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Updated: Jul 10, 2026
Whole Mount Immunofluorescent Staining of the Neonatal Mouse Retina to Investigate Angiogenesis In vivo
Published on: July 9, 2013
An immunofluorescence-histochemistry study of the Nogo receptor in the rat retina during postnatal development
Xiaolei Yin1, Chunlin Chen, Rongdi Yuan
1Department of Ophthalmology, Daping Hospital, Third Military Medical University, 10, Changjiang Zhilu, Daping, Chongqing, 400042, China. yinxiaolei971221@163.com
Abstract:
We investigated expression of the Nogo receptor (NgR) in the retina of rats between postnatal day 0 (PND 0) and adulthood (PND 63). Frozen sections of eyeball were prepared from each of six rats on PND 0, PND 3, PND 7, PND 14, PND 21, PND 35, PND 49, and PND 63. Immunofluorescence-histochemistry and laser-confocal microscopy were used to observe the expression of NgR protein. NgR is positive in the retina at all stages. It was distributed around ganglion cell nuclei and disposed linearly in the optic nerve. The NgR was expressed in rat retina at the time of birth and was present throughout postnatal development.
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