Non-intuitive separation of vanilla compounds using rapid resolution high-performance liquid chromatography
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Updated: Jul 10, 2026

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
Published on: June 1, 2017
Jean-Paul Larcinese1, Fabrice Avaltroni, Valéry Normand
1FIRMENICH SA, P.O. Box 148, CH-1217 MEYRIN 2 (Geneva), Switzerland.
Abstract:
A method is presented for modeling the retention peak migration in rapid resolution high-performance liquid chromatography (HPLC) depending on experimental parameter values. It allows time reduction on the determination of the experimental conditions for optimal resolution (especially for untrained chromatographers). Separation for 18 species present in a conventional vanilla formulation was not possible in a single chromatogram, due to a systematic error in defining single peak migration with the usual assumptions. This was achieved by the means of two runs under different experimental conditions. Prediction of the peak inversion for quantitation purposes in a given mixture is now possible and can help to avoid misidentification on set-ups with UV-ELSD or other non-specific detectors.
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