Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Studies VII: Vascular Imaging01:19

Imaging Studies VII: Vascular Imaging

DefinitionRenal angiography, also known as renal arteriography, is an imaging technique used to obtain a comprehensive view of blood flow and the vascular structure of blood vessels in the kidneys and surrounding areas.PurposeRenal angiography detects blood vessel abnormalities in the kidneys, such as aneurysms, stenosis, thrombosis, vascular tumors, and renal artery stenosis. It evaluates kidney function and guides interventional treatments like angioplasty or stent placement.Pre-Procedure...
Imaging Studies II: Ultrasonography01:24

Imaging Studies II: Ultrasonography

IntroductionUltrasonography, or renal ultrasound, is a noninvasive medical imaging technique that uses high-frequency sound waves to visualize the kidneys, ureters, bladder, and surrounding tissues.Indications for Urinary System UltrasonographyUrinary system ultrasonography is indicated in various clinical scenarios, such as:Kidney Stones (Urolithiasis): To detect and monitor the size and presence of kidney or urinary tract stones.Hydronephrosis: To assess the dilation of the renal pelvis and...
Imaging Studies I: Kidney, Ureter, and Bladder Studies01:28

Imaging Studies I: Kidney, Ureter, and Bladder Studies

Kidney, Ureter, and Bladder (KUB) StudiesKidney, Ureter, and Bladder (KUB) studies are standard diagnostic imaging procedures used to assess the anatomy of the urinary system. They are commonly utilized for patients experiencing abdominal pain or urinary symptoms. By using a simple X-ray of the abdomen, KUB studies can reveal structural and pathological abnormalities within the kidneys, ureters, and bladder. These studies are particularly valuable in diagnosing kidney stones, urinary...
Imaging Studies V: Intravenous Urography and Retrograde Pyelography01:22

Imaging Studies V: Intravenous Urography and Retrograde Pyelography

IntroductionIntravenous Urography (IVU) and Retrograde Pyelography (RP) are important diagnostic imaging techniques used to evaluate the urinary system. These methods help identify structural abnormalities, obstructions, and functional issues in the kidneys, ureters, and bladder. Both procedures use iodine-based contrast media to enhance the visibility of urinary tract structures on X-ray images, though they differ in their methods and indications.1. Intravenous Urography (IVU)Intravenous...
Drug Dosing in Renal Diseases: Measurement of Glomerular Filtration Rate01:25

Drug Dosing in Renal Diseases: Measurement of Glomerular Filtration Rate

The glomerular filtration rate (GFR) is a critical indicator of kidney health, reflecting how well the kidneys filter blood. Changes in GFR can signal potential kidney impairment, necessitating accurate measurement methods to monitor kidney function effectively.Various molecules can serve as markers for GFR measurement, with the ideal marker meeting several specific criteria. It must freely filter at the glomerulus, avoid reabsorption or secretion by the renal tubules, remain unmetabolized, not...
Acute Kidney Injury IV: Diagnostic Studies and Prevention01:30

Acute Kidney Injury IV: Diagnostic Studies and Prevention

Accurate diagnosis and effective prevention are critical in managing Acute Kidney Injury (AKI), which is linked to high mortality rates ranging from 10% to 80%. Timely recognition of at-risk patients and careful monitoring can significantly reduce the likelihood of kidney damage.Diagnostic Assessments:The diagnostic process starts with a comprehensive medical history to identify prerenal, intrarenal, and postrenal causes.Prerenal causes, such as dehydration, hypotension, or blood loss, should...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Treatment of Autosomal Dominant Polycystic Kidney Disease: Integrating Clinical Practice Guidelines, Patient Perspectives, and Real-World Effectiveness.

Journal of the American Society of Nephrology : JASN·2026
Same author

A new Jun amino-terminal kinase inhibitor, KRev-202, inhibits rat ischemic acute injury and the progression to renal fibrosis.

Frontiers in pharmacology·2026
Same author

Participant Perceptions of Increasing Water Intake in Polycystic Kidney Disease.

Kidney international reports·2025
Same author

A randomized controlled trial of intravenous immunoglobulin vs standard of care for the treatment of chronic active antibody-mediated rejection in kidney transplant recipients.

Kidney international·2025
Same author

Response to Early Detection Matters: Bridging Evidence and Practice, a Call for Enhanced Cardiovascular Screening in ADPKD.

Kidney international reports·2025
Same author

Validating the SONG-PKD Pain Instrument, a Core Outcome Measure for Pain in ADPKD.

Kidney international reports·2025

Related Experiment Video

Updated: Jul 10, 2026

Point-of-Care Kidney and Genitourinary Ultrasound in Adults: Image Acquisition
03:19

Point-of-Care Kidney and Genitourinary Ultrasound in Adults: Image Acquisition

Published on: June 21, 2024

Quantification of renal pathology by image analysis.

Gopala K Rangan1, Greg H Tesch

  • 1Centre for Transplant and Renal Research, Westmead Millennium Institute, The University of Sydney, Sydney, New South Wales, Australia. g.rangan@wmi.usyd.edu.au

Nephrology (Carlton, Vic.)
|November 13, 2007
PubMed
Summary

Computer image analysis accurately quantifies kidney lesions using Image J software. This guide details methods for analyzing digital microscopy images to assess various renal pathologies and scarring.

More Related Videos

In Vivo, Percutaneous, Needle Based, Optical Coherence Tomography of Renal Masses
09:31

In Vivo, Percutaneous, Needle Based, Optical Coherence Tomography of Renal Masses

Published on: March 30, 2015

Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes
11:47

Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes

Published on: March 4, 2022

Related Experiment Videos

Last Updated: Jul 10, 2026

Point-of-Care Kidney and Genitourinary Ultrasound in Adults: Image Acquisition
03:19

Point-of-Care Kidney and Genitourinary Ultrasound in Adults: Image Acquisition

Published on: June 21, 2024

In Vivo, Percutaneous, Needle Based, Optical Coherence Tomography of Renal Masses
09:31

In Vivo, Percutaneous, Needle Based, Optical Coherence Tomography of Renal Masses

Published on: March 30, 2015

Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes
11:47

Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes

Published on: March 4, 2022

Area of Science:

  • Nephrology
  • Pathology
  • Biomedical Image Analysis

Background:

  • Accurate quantification of kidney pathological lesions is crucial for understanding disease progression and treatment efficacy.
  • Traditional methods for lesion assessment can be subjective and time-consuming.
  • Advances in computer image analysis offer objective and efficient quantification of histological features.

Purpose of the Study:

  • To provide a detailed description of basic techniques for quantifying kidney pathological lesions in digital light microscopy images.
  • To demonstrate the application of Image J software for objective histological analysis.
  • To facilitate the evaluation of diverse renal pathologies using standardized image analysis methods.

Main Methods:

  • Utilizing Image J software for digital image analysis of kidney histology.
  • Implementing techniques such as measurement calibration, scale application, object size and thickness assessment.
  • Employing cell counting, point counting, and stained area analysis.

Main Results:

  • The described methods enable precise quantification of glomerular hypertrophy.
  • Accurate assessment of tubular and vascular injury is achievable.
  • Quantification of inflammatory cell and myofibroblast accumulation, extracellular matrix deposition, and renal scarring is facilitated.

Conclusions:

  • Image J software provides a powerful and accessible tool for quantifying kidney pathological lesions.
  • These image analysis techniques enhance the accuracy and objectivity of renal pathology assessment.
  • Standardized quantification aids in evaluating disease severity and therapeutic responses in kidney diseases.