Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immunoglobulin A: interaction with complement, phagocytic cells and endothelial cells.

W M Bogers1, R K Stad, L A van Es

  • 1Department of Nephrology, University Hospital, Leiden, The Netherlands.

Complement and Inflammation
|January 1, 1991
PubMed
Summary

Immunoglobulin A (IgA) can trigger inflammation by activating complement (C) and phagocytic cells. Understanding IgA interactions is key to diseases like IgA nephropathy, with new insights into IgA clearance mechanisms.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

HLA A, B, DR and endothelial-specific antigens in kidney-graft rejection.

Immunology today·2014
Same author

Genetic associations in diabetic nephropathy: a meta-analysis.

Diabetologia·2010
Same author

GMP-17-positive T-lymphocytes in renal tubules predict progression in early stages of IgA nephropathy.

Kidney international·2008
Same author

Renal disease in relatives of Indo-Asian Type 2 diabetic patients with end-stage diabetic nephropathy.

Diabetologia·2003
Same author

Increased end-stage diabetic nephropathy in Indo-Asian immigrants living in the Netherlands.

Diabetologia·2002
Same author

Risk factors of cyclosporine nephrotoxicity after conversion from Sandimmune to Neoral.

Clinical nephrology·2001

Area of Science:

  • Immunology
  • Complement System
  • Cellular Biology

Background:

  • Deposits of Immunoglobulin A (IgA) and complement (C) suggest IgA's role in inflammation.
  • Understanding IgA interactions with C and phagocytic cells is crucial for inflammatory disease research.

Purpose of the Study:

  • To investigate the inflammatory potential of IgA.
  • To elucidate the mechanisms of IgA-mediated complement activation and clearance.
  • To explore IgA interactions with phagocytic and endothelial cells.

Main Methods:

  • Demonstration of polymeric human serum IgA activating the alternative C pathway.
  • Analysis of IgA molecular composition effects on C activation using rat monoclonal IgA.
  • Investigation of IgA immune complex (IC) clearance by Kupffer cells and endothelial cells in a rat model.

Related Experiment Videos

Main Results:

  • Polymeric IgA (pIgA) and dimeric IgA (dIgA) activate the alternative C pathway, with the F(ab')2 portion being key.
  • Monomeric IgA (mIgA) shows poor C-activating potential.
  • IgA-IC clearance involves Kupffer cells and C, with endothelial cells playing a role in their absence.

Conclusions:

  • IgA possesses significant inflammatory potential through C activation and interaction with phagocytic cells.
  • Kupffer cells and C are vital for IgA-IC clearance, with endothelial cells offering an alternative pathway.
  • Findings contribute to understanding IgA nephropathy and Henoch-Schönlein purpura pathogenesis.