Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Biomedical polymers differ in their capacity to activate complement.

J Janatova1, A K Cheung, C J Parker

  • 1Department of Pathology, University of Utah School of Medicine, Salt Lake City.

Complement and Inflammation
|January 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Effects of Menstrual Cycle Phase on Gastrointestinal Responses to a Simulated Football Match.

European journal of sport science·2026
Same author

Benefits and risks of intensive blood-pressure lowering in advanced chronic kidney disease.

Journal of internal medicine·2018
Same author

Long-Term Follow-Up Results following Elective Treatment of Unruptured Intracranial Aneurysms with the Pipeline Embolization Device.

AJNR. American journal of neuroradiology·2015
Same author

Visit-to-visit systolic blood pressure variability and outcomes in hemodialysis.

Journal of human hypertension·2013
Same author

Usability of Geographic Information: current challenges and future directions.

Applied ergonomics·2012
Same author

Leukogram abnormalities in gnotobiotic pigs infected with porcine circovirus type 2.

Veterinary microbiology·2011
Same journal

Computer-assisted kinetic assay for quantification of total complement activity.

Complement and inflammation·1991
Same journal

HLA complement markers in Italian narcoleptic patients with special emphasis on BfF subtyping.

Complement and inflammation·1991
Same journal

Sarcoidosis and major histocompatibility complex genes with special emphasis on BF F subtypes.

Complement and inflammation·1991
Same journal

Clinical manifestations in humans of combined C7 and C4 deficiency associated with low levels of C2, C8, and C9.

Complement and inflammation·1991
Same journal

Bibliography of complement research for 1989.

Complement and inflammation·1991
Same journal

Differences in the complement activation induced by preformed and nascent immune complexes.

Complement and inflammation·1991
See all related articles

Biomaterial classification based on fluid-phase C3a concentration is inaccurate. Complement activation by polymers depends on complex surface interactions, not just binding sites, necessitating further research into molecular mechanisms.

Area of Science:

  • Biomaterials Science
  • Immunology
  • Polymer Chemistry

Background:

  • Complement activation by biomedical polymers is traditionally assessed by fluid-phase C3a concentration.
  • This method has led to inaccurate classifications of biomaterials like hemodialysis membranes.
  • Certain polymers bind significant C3a, skewing results.

Purpose of the Study:

  • To re-evaluate the conventional methods for assessing complement activation by biomedical polymers.
  • To investigate the factors determining a polymer's capacity to activate complement.
  • To understand the molecular mechanisms of complement activation on artificial surfaces.

Main Methods:

  • Comparative analysis of complement activation by cuprophane and polyacrylonitrile membranes.

Related Experiment Videos

  • Assessment of C3 binding (activated and unactivated) to biomaterials.
  • Evaluation of factor B and factor H binding to biomaterials independently of complement activation.

    • Main Results:

    • Polymer's complement activation capacity is not solely determined by surface covalent binding sites.
    • Biomaterials can activate C3 efficiently even without hydroxyl/amino groups.
    • Factor B and H binding to surfaces can be independent of complement activation and C3 presence.

    Conclusions:

    • Fluid-phase C3a measurement is an unreliable sole criterion for classifying biomaterial complement activators.
    • Surface interactions governing C3 convertase formation on artificial surfaces differ from biological membranes.
    • Further research is crucial to elucidate the molecular mechanisms of C3 and C5 activation on biomedical polymer surfaces.