Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Computer-assisted kinetic assay for quantification of total complement activity.

M Abbal1, J Tkaczuk, C Praud

  • 1Laboratoire Central d'Immunologie, CHU Toulouse Rangueil, France.

Complement and Inflammation
|January 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Short cold exposures during incubation and postnatal cold temperature affect performance, breast meat quality, and welfare parameters in broiler chickens.

Poultry science·2020
Same author

Beta-carotene preferentially regulates chicken myoblast proliferation withdrawal and differentiation commitment via BCO1 activity and retinoic acid production.

Experimental cell research·2017
Same author

Reference values for T, B and NK human lymphocyte subpopulations in adults.

Data in brief·2017
Same author

Influence of age, sex and HCMV-serostatus on blood lymphocyte subpopulations in healthy adults.

Cellular immunology·2017
Same author

Methodological aspects of minimal residual disease assessment by flow cytometry in acute lymphoblastic leukemia: A French multicenter study.

Cytometry. Part B, Clinical cytometry·2014
Same author

Cyclic variations in incubation conditions induce adaptive responses to later heat exposure in chickens: a review.

Animal : an international journal of animal bioscience·2014
Same journal

HLA complement markers in Italian narcoleptic patients with special emphasis on BfF subtyping.

Complement and inflammation·1991
Same journal

Sarcoidosis and major histocompatibility complex genes with special emphasis on BF F subtypes.

Complement and inflammation·1991
Same journal

Clinical manifestations in humans of combined C7 and C4 deficiency associated with low levels of C2, C8, and C9.

Complement and inflammation·1991
Same journal

Biomedical polymers differ in their capacity to activate complement.

Complement and inflammation·1991
Same journal

Bibliography of complement research for 1989.

Complement and inflammation·1991
Same journal

Differences in the complement activation induced by preformed and nascent immune complexes.

Complement and inflammation·1991
See all related articles

A new semiautomated kinetic technique offers a simple method for complement activity assays. This computer-assisted approach provides accurate and efficient analysis of patient sera, suitable for routine laboratory use.

Area of Science:

  • Clinical chemistry
  • Immunology
  • Biophysical chemistry

Background:

  • Complement activity assays are crucial for diagnosing immune deficiencies and monitoring treatment.
  • Traditional methods can be time-consuming and require specialized expertise.
  • There is a need for efficient, accurate, and accessible complement activity assays.

Purpose of the Study:

  • To develop and validate a semiautomated kinetic technique for complement activity assay.
  • To compare the performance of the new technique with a standard method.
  • To assess the feasibility of the technique for routine laboratory application.

Main Methods:

  • Hemolysis of sensitized red blood cells in a thermostated microflow cell connected to a spectrophotometer and computer.

Related Experiment Videos

  • Computerized control, display, and analysis of assay phases.
  • Comparison with Kabat and Mayer's method using 221 patient sera.

    • Main Results:

    • High correlation (regression coefficient 0.94) between the new technique and the standard method.
    • Similar results for samples with complement fraction deficiencies or in vitro activation.
    • Excellent precision: coefficient of variation ~1% within series and <3% between series.
    • High throughput: up to 30-40 samples per hour.

    Conclusions:

    • The semiautomated kinetic technique is a reliable and reproducible method for complement activity assay.
    • The technique is user-friendly and suitable for routine, nonspecialized laboratories.
    • This method offers an efficient alternative for assessing complement system function.