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Updated: Jul 10, 2026

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
Published on: July 8, 2025
Design of dual-labeled oligonucleotide probes for SNPs genotyping
Yoshio Saito1, Subhendu Sekhar Bag, Satoshi Kodate
1Department of Marerials Chemistry and Engineering, School of Engineering, Nihon University, Koriyama, Fukushima 963-8642, Japan. saito@mech.ce.nihon-u.ac.jp
Abstract:
Our effort in designing base-discriminating fluorescence nucleosides (BDF), leads us to develop dual-labeled oligonucleotide probe in which the BDF nucleoside, (Py)U/(2-Ant)U act as the donor separated by a defined base pair distance from the acceptor, fluorescein, attached to 5'-end of the probe. Thus, a longer wavelength emission from acceptor might allow the probe to be used for SNP typing in chip based detection technology or in cell.
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