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Updated: Jul 10, 2026
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Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization (LNA flow-FISH): a Method for Bacterial Small RNA Detection
Published on: January 10, 2012
Novel applications of locked nucleic acids
Rakesh N Veedu1, Birte Vester, Jesper Wengel
1Nucleic Acid Center, University of Southern Denmark, Campusvej 55, DK - 5230 Odense M, Denmark.
Nucleic Acids Symposium Series (2004)
|November 22, 2007
Summary
Locked Nucleic Acid (LNA) nucleoside triphosphates are substrates for DNA polymerases in primer extension. DNA 9oNm polymerase is optimal for PCR amplification using LNA-A nucleotides.
Area of Science:
- Biochemistry
- Molecular Biology
- Nucleic Acid Chemistry
Background:
- Locked Nucleic Acid (LNA) is a modified nucleic acid with enhanced binding affinity.
- Investigating the substrate properties of LNA nucleoside triphosphates is crucial for their application in molecular biology.
Purpose of the Study:
- To evaluate the substrate capabilities of Locked Nucleic Acid (LNA) nucleoside triphosphates.
- To determine the optimal DNA polymerase for primer extension and PCR using LNA nucleotides.
Main Methods:
- Synthesis of Locked Nucleic Acid (LNA) nucleoside triphosphates.
- Primer extension assays with various DNA polymerases.
- Polymerase Chain Reaction (PCR) experiments utilizing LNA nucleotides.
Main Results:
- Phusion High Fidelity DNA polymerase and 9oNm DNA polymerase efficiently utilize LNA nucleoside 5'-triphosphates in primer extension.
- DNA 9oNm polymerase demonstrated superior performance for PCR amplification with LNA-A nucleotide.
Conclusions:
- LNA nucleoside triphosphates are viable substrates for specific DNA polymerases in primer extension.
- DNA 9oNm polymerase is recommended for PCR applications involving LNA-A for efficient amplification.
