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Updated: Jan 5, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
Published on: April 4, 2025
Effects of cosolutes on the thermodynamic stability of parallel DNA duplex and triplex
Kaori Nakamura1, Hisae Karimata, Tatsuo Ohmichi
1Department of Chemistry, Faculty of Science and Engineering, Konan University 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan.
Abstract:
We investigated molecular crowding effects on the thermodynamic stability of Hoogsteen and Watson-Crick base pairs in an intramolecular duplex and triplex. The melting temperature (Tm) of Hoogsteen base pair formations in the triplex and the duplex increased 3.7 degrees C and 3.2 degrees C, respectively, by adding 20 wt% PEG 200. On the other hand, the Tm of Watson-Crick base pair formations in the triplex and the duplex decreased 5.7 degrees C and 5.2 degrees C, respectively. These results suggested that molecular crowding conditions generally stabilized and destabilized Hoogsteen and Watson-Crick base pairs, respectively, even in the different DNA structures.
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