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Updated: Jul 10, 2026

Chemical Triphosphorylation of Oligonucleotides
Published on: June 2, 2022
Ce(IV)-catalyzed site-selective DNA hydrolysis using tris- and tetrakismethylenephosphonate-modified oligonucleotides
Tuomas Lönnberg1, Yuta Suzuki, Makoto Komiyama
1RCAST, The University of Tokyo, 4-6-1, Komaba, Meguro-Ku, Tokyo 153-8904, Japan. tuanlo@utu.fi
Abstract:
To improve the recruitment of the catalytically active Ce(IV) ion to the DNA cleavage site in the ARCUT (Artificial Restriction DNA Cutter) system, two phosphonate ligands, nitrilotris(methylenephosphonic acid) (NTP) and N,N,N',N'-ethylenediaminetetra-(methylenephosphonic acid) (EDTP), have been linked to the 5'- or 3'-end of oligonucleotides complementary to the target DNA. Compared to their counterparts bearing a terminal monophosphate, these new modified oligonucleotides exhibit a modest to significant increase in the cleavage activity, depending on the target.
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