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Updated: Jul 10, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
Published on: September 15, 2021
Photocontrol of site-selective RNA scission
Keita Tanaka1, Yoji Yamamoto, Akinori Kuzuya
1Research Center for Advanced Science and Techonology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8904 Japan.
Abstract:
Novel photoresponsive systems for site-selective RNA scission have been prepared by combining metal ions with DNA bearing an azobenzene adjacent to an acridine. The modified DNA forms a heteroduplex with the substrate RNA, and selectively activates the phosphodiester linkages in front of the acridine, only when the azobenzene is isomerized into cis form by UV irradiation. Trans-azobenzene, on the other hand, effectively suppresses the reaction. As a result, 1.7-2.4-fold acceleration of the cleavage was achieved only with 3 min UV irradiation.
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