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Fluorescence emission detection of single-nucleotide polymorphisms by a naphthyridine-benzofurazan conjugate

Hiroyuki Satake1, Seiichi Nishizawa, Norio Teramae

  • 1Department of Chemistry, Graduate School of Science, Tohoku University, and CREST, Japan Science and Technology Agency (JST), Aoba-ku, Sendai 980-8578, Japan.

Nucleic Acids Symposium Series (2004)
|November 22, 2007
PubMed

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...