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Updated: Jul 10, 2026

Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry
Published on: April 11, 2022
A facile method for the detection of RNA-polypeptide interactions by MALDI-TOF mass spectrometry
Maki Sugaya1, Kazuo Harada, Akira Katoh
1Department of Materials and Life Science, Faculty of Science and Technology, Seikei University, Musashino, Tokyo 180-8633, Japan.
Abstract:
The development of soft ionization methods for mass spectrometry has made possible the analysis of large and relatively unstable biological samples. However, the analysis of RNA-polypeptide interactions has been hampered by the low detection sensitivity to RNA and the resulting RNA-polypeptide complexes. In this study, we attempted the detection of RNA-polypeptide complexes by MALDI-TOF MS, focusing on the disappearance of the peptide signal upon binding to the RNA. It was demonstrated that specifically RNA-binding peptides could be identified from a mixture of peptides, possibly enabling high throughput screening of RNA-binding polypeptides.
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