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Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
Published on: November 1, 2019
Screening of a combinatorial library reveals peptide-based catalysts for phosphorester cleavage in water
Carsten Schmuck1, Jürgen Dudaczek
1University of Würzburg, Institute of Organic Chemistry, Am Hubland, 97074 Würzburg, Germany. schmuck@chemie.uni-wuerzburg.de
Organic Letters
|November 23, 2007
Abstract:
A combinatorial library of 625 octapeptides was screened for their efficiency to catalyze phosphorester cleavage in water. Octapeptides such as 1c, which contains a catalytically active histidine and two cationic residues (Gua) as potential anion-binding sites, show substrate hydrolysis in water. Metal-free catalysis with a rate enhancement of up to a factor of 175 over the uncatalyzed background reaction is observed.
