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Related Experiment Video

Updated: Jul 9, 2026

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells
09:28

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells

Published on: December 13, 2010

Modular tissue engineering: fabrication of a gelatin-based construct.

Alison P McGuigan1, Michael V Sefton

  • 1Department of Chemical Engineering and Applied Chemistry, University of Toronto, Canada.

Journal of Tissue Engineering and Regenerative Medicine
|November 27, 2007
PubMed
Summary

Researchers developed modular tissue-engineered constructs from gelatin for blood vessel applications. Glutaraldehyde crosslinking preserved structure but killed cells, highlighting the need for non-toxic methods to create viable constructs.

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Area of Science:

  • Biomaterials Engineering
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Modular tissue-engineered constructs require perfusable channels for nutrient delivery.
  • Gelatin offers a suitable substrate for endothelial cells but lacks mechanical stability.
  • Crosslinking is necessary to maintain construct integrity in physiological conditions.

Purpose of the Study:

  • To fabricate and assess modular tissue-engineered constructs using gelatin.
  • To evaluate the suitability of gelatin as a scaffold for endothelial cell seeding and construct assembly.
  • To investigate the impact of glutaraldehyde crosslinking on construct stability and cell viability.

Main Methods:

  • Gelatin modules containing HepG2 cells or spheroids were fabricated via sieving.

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Last Updated: Jul 9, 2026

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells
09:28

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells

Published on: December 13, 2010

Fabrication of Myogenic Engineered Tissue Constructs
13:43

Fabrication of Myogenic Engineered Tissue Constructs

Published on: May 1, 2009

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration
09:23

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Published on: June 16, 2015

  • Bovine aortic endothelial cells were seeded to form a surface layer.
  • Constructs were assembled in a flow circuit to measure perfusion characteristics.
  • Glutaraldehyde was used to crosslink gelatin modules to prevent dissolution.

    • Main Results:

    • Endothelial cell lining was established within one week.
    • Constructs demonstrated negligible compaction under high flow rates.
    • Glutaraldehyde crosslinking prevented dissolution but caused significant cell death.
    • A non-toxic crosslinking strategy is needed for viable cell-containing constructs.

    Conclusions:

    • Modular gelatin constructs can be fabricated with perfusable channels.
    • Current glutaraldehyde crosslinking methods are incompatible with cell viability.
    • Further research is required to develop non-toxic crosslinking strategies for functional tissue-engineered constructs.