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Updated: Jul 9, 2026

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization
Published on: June 28, 2012
[Comparative study on polytene chromosomes of two isolates of Simulium quinquestriatus]
Xiao-jun Wen1, Jing Wei, Han-bing Chen
1Department of Biology, Guiyang Medical College, Guiyang 550004, China.
Abstract:
The salivary glands were exposed and isolated from the larvae of Simulium quinquestriatus and stained in carbol fuchsin, squashed between slide and coverslide. Slides were examined and photographed under microscope to measure the polytene chromosomes. Systematic analysis was made. Results indicated that the number of the polytene chromosomes of both isolates is three. The main characteristic chromosomal structures are homologized. Only the banding types of II L are different.
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