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MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
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Metal sensing by RNA in bacteria: exception or rule?
1AG Bakteriengenetik, Friedrich-Schiller-Universität Jena, Philosophenweg 12, D-07743 Jena, Germany. Sabine.Brantl@uni-jena.de
ACS Chemical Biology
|November 29, 2007
Summary
RNA-based metal ion sensing in bacteria challenges previous assumptions. A newly discovered riboswitch in Gram-positive bacteria indicates this mechanism is widespread, expanding our understanding of bacterial gene regulation.
Area of Science:
- Bacterial molecular biology
- RNA-based gene regulation
Background:
- Previously, bacteria were thought to exclusively use proteins for intracellular metal ion concentration control.
- This understanding was challenged by the discovery of the first Mg2+-sensing riboswitch in Salmonella.
Purpose of the Study:
- To investigate the prevalence and mechanism of RNA-based metal ion sensing in bacteria.
- To characterize a newly identified metal-ion-sensing riboswitch.
Main Methods:
- Discovery and in-depth characterization of a novel riboswitch.
- Analysis of riboswitch presence in Gram-positive bacteria.
Main Results:
- A second metal-ion-sensing riboswitch was identified.
- This riboswitch appears to be ubiquitously present in Gram-positive bacteria.
Conclusions:
- RNA-based metal ion sensing is likely a widespread mechanism in bacteria.
- This finding necessitates a revision of our understanding of bacterial metal ion homeostasis and gene regulation.
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