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Cloning, expression, purification, characterization, crystallization and x-ray diffraction of bifunctional pyrimidine
Daopeng Yuan1, Qihai Wang, Wei Gao
1College of Science, Beijing Forestry University, Beijing 100083, , China.
Protein and Peptide Letters
|November 30, 2007
Abstract:
Bifunctional pyrimidine deaminase/reductase (RibD) plays an important role during riboflavin biosynthesis in many microorganisms. The 40.4 kDa RibD from Shigella flexneri 2a has been cloned, expressed, purified and characterized. Three Crystals of RibD have been obtained by the hanging-drop technique at 291 K using PEG 20k or NaCl as precipitant. The RibD crystal using PEG 20k as precipitant diffracted to 2.5A.
