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[Effect of LIPUS on the cellular behavior of human primary macrophages]
Yi-Ping Li1, Shao-Xia Zhou, Schmelz Andreas
1Department of Pathology, Basic Medical School, Southeast University, Nanjing 210009, China. lypzzm@yahoo.com.cn
Aim:
To explore the effect of low intensity pulsed ultrasound (LIPUS) on the biological behavior of macrophages to provide some theoretical foundations for its clinical application.
Methods:
Human primary macrophages were obtained by using modified Ficoll-paque density gradient centrifugation combined with adherent method. The phagocytosis of macrophages was analyzed using fluorescein-labelled E.coli. The expression of the secreted proteins was determined by proteins array and the secretion of metalloproteinases (MMPs) was examined by gelatin zymography. The focal complexes and extracellular matrix metalloproteinase inducer (CD147) were detected by fluorescence microscopy and the phosphorylation of Src and ERKs was examined by Western blot.
Results:
The human primary macrophages were successfully isolated and cultured in vitro. LIPUS accelerated the macrophages to phagocytose E.coli, promoted the protein expression of CD147 and MMPs, increased the level of protein tyrosine phosphorylation, and induced the formation of focal complexes and the phosphorylation of Src and ERKs.
Conclusion:
LIPUS may up-regulate the phagocytosis and secretion of human primary macrophages through the activation of Src and ERKs.
Insights
Low intensity pulsed ultrasound (LIPUS) enhances macrophage phagocytosis and protein secretion. This effect is mediated by activating Src and extracellular signal-regulated kinases (ERKs), providing theoretical support for LIPUS clinical use.
Area of Science:
- Cell Biology
- Biophysics
- Immunology
Background:
- Low intensity pulsed ultrasound (LIPUS) is a non-invasive therapeutic modality.
- Macrophages play crucial roles in immunity and tissue repair.
- Understanding LIPUS effects on macrophage behavior is vital for clinical applications.
Purpose of the Study:
- To investigate the impact of LIPUS on the biological behavior of human primary macrophages.
- To elucidate the underlying molecular mechanisms of LIPUS-induced macrophage activation.
- To provide theoretical foundations for the clinical application of LIPUS in modulating macrophage function.
Main Methods:
- Isolation and in vitro culture of human primary macrophages.
- Assessment of macrophage phagocytosis using fluorescein-labeled E. coli.
- Analysis of secreted proteins via protein array, metalloproteinases (MMPs) by gelatin zymography, CD147 expression, focal complex formation, and Src/ERK phosphorylation by Western blot.
Main Results:
- Successful isolation and culture of human primary macrophages.
- LIPUS significantly accelerated E. coli phagocytosis by macrophages.
- LIPUS promoted CD147 and MMP expression, induced focal complex formation, and enhanced Src and ERK phosphorylation.
Conclusions:
- LIPUS up-regulates the phagocytic capacity of human primary macrophages.
- LIPUS enhances the secretion of proteins, including MMPs, from macrophages.
- The observed effects are likely mediated through the activation of Src and ERK signaling pathways.
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