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TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
Published on: June 8, 2020
Tandem mass spectrometry in physiology.
Trairak Pisitkun1, Jason D Hoffert, Ming-Jiun Yu
1Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA.
Physiology (Bethesda, Md.)
|December 13, 2007
Summary
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) enables "shotgun" proteomics for protein identification without purification. Advances in quantification and phosphoproteomics make LC-MS/MS vital for cell physiology research.
Area of Science:
- Biochemistry
- Proteomics
- Cell Biology
Background:
- "Shotgun" proteomics using liquid chromatography-tandem mass spectrometry (LC-MS/MS) allows protein identification from complex mixtures.
- Prior purification of proteins is not required for LC-MS/MS analysis.
- Recent advancements have expanded the utility of LC-MS/MS.
Purpose of the Study:
- To highlight the capabilities of LC-MS/MS in modern biological research.
- To emphasize the role of LC-MS/MS in studying cell physiology.
Main Methods:
- Tandem mass spectrometry coupled with liquid chromatography (LC-MS/MS).
- Techniques include "shotgun" proteomics, quantitative analysis, and phosphoproteomic analysis.
- Targeted LC-MS/MS using multiple reaction monitoring (MRM) is also employed.
Main Results:
- LC-MS/MS facilitates direct protein identification from complex biological samples.
- Progress in quantification and phosphoproteomic analysis enhances its application.
- Targeted LC-MS/MS provides specific protein quantification.
Conclusions:
- LC-MS/MS is a powerful and versatile tool for biological research.
- Its applications are crucial for understanding complex cellular processes and physiology.
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