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Updated: Jul 9, 2026

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins
Published on: November 1, 2024
Resolution and sensitivity aspects of ultrafast J-resolved 2D NMR spectra
Patrick Giraudeau1, Serge Akoka
1Centre National de la Recherche Scientifique, Laboratoire d'Analyse Isotopique et Electrochimique de Métabolismes, Université de Nantes, UMR 6006, 2 rue de la Houssinière, Nantes Cedex 03, France. patrick.giraudeau@univ-nantes.fr
Abstract:
Recent ultrafast techniques enable nD NMR spectra to be obtained in a single scan. However, resolution enhancement in the ultrafast domain leads to important sensitivity losses and lineshape distortions. In order to understand better resolution and spatial encoding aspects of continuous phase-encoding schemes, a theoretical and experimental comparison of different excitation patterns is carried out. Molecular diffusion appears to be the main cause of signal-to-noise ratio decrease, and a multi-echo excitation scheme is proposed to limit its effects when a good resolution is needed. Results obtained on 2D J-resolved spectra are presented.
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