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Updated: Jul 9, 2026
09:26
FLIM-FRET Measurements of Protein-Protein Interactions in Live Bacteria.
Published on: August 25, 2020
Photon-counting technique for rapid fluorescence-decay measurement.
S D Pack1, M W Renfro, G B King
1Flame Diagnostics Laboratory, School of Mechanical Engineering, Purdue University, West Lafayette, Indiana 47907-1288, USA.
Optics Letters
|December 19, 2007
Summary
A new laser-induced fluorescence triple-integration method (LIFTIME) enables rapid, continuous fluorescence lifetime measurements. This technique allows for real-time monitoring of species concentrations in turbulent flames.
Area of Science:
- Chemical Physics
- Spectroscopy
- Fluid Dynamics
Background:
- Accurate measurement of fluorescence lifetimes is crucial for understanding chemical species.
- Existing methods can be limited in speed and applicability to dynamic environments like turbulent flames.
Purpose of the Study:
- To introduce and validate a novel method for rapid, continuous fluorescence lifetime measurements.
- To enable real-time monitoring of species concentrations in challenging environments.
Main Methods:
- Development of the laser-induced fluorescence triple-integration method (LIFTIME).
- Convolution of LIFTIME with picosecond time-resolved laser-induced fluorescence using a high-repetition-rate mode-locked laser.
- Application and verification using standard fluorescence media: diphenyloxazole (PPO) and quinine sulfate monohydrate (QSM).
Main Results:
- Demonstrated capability for rapid, continuous fluorescence lifetime measurements.
- Achieved fluorescence lifetime measurements at sampling rates up to 500 Hz.
- Maintained less than 10% uncertainty (1 sigma) in measurements.
Conclusions:
- The LIFTIME technique provides a significant advancement in fluorescence lifetime measurement speed and continuity.
- This method is suitable for monitoring instantaneous species concentrations in turbulent flames.
- Validated accuracy and reliability of LIFTIME with known fluorescence standards.
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