Related Experiment Videos
Rational design of purification processes for recombinant proteins
J A Asenjo1, J Parrado, B A Andrews
1Biochemical Engineering Laboratory, University of Reading, England.
Annals of the New York Academy of Sciences
|December 27, 1991
Summary
This study reviews rational design for recombinant protein purification processes. It highlights challenges and introduces an expert system for optimizing large-scale separation sequences using mathematical modeling.
Area of Science:
- Biotechnology
- Biochemical Engineering
- Protein Chemistry
Background:
- Recombinant protein production requires efficient purification strategies.
- Challenges in protein separation and purification are amplified for recombinant proteins.
- Rational process design is crucial for scalable and cost-effective purification.
Purpose of the Study:
- To discuss recent advancements in the rational design of purification processes for recombinant proteins.
- To review key issues in protein separation and purification process design.
- To introduce an expert system for optimizing large-scale protein purification.
Main Methods:
- Physicochemical characterization of target proteins and contaminants.
- Application of rigorous mathematical modeling and process simulation.
- Development and utilization of an expert system for process design and optimization.
Main Results:
- Identification of challenges specific to recombinant protein purification.
- Demonstration of mathematical modeling and simulation for process design.
- Development of an expert system to aid in selecting optimal separation sequences.
Conclusions:
- Rational design principles are essential for effective recombinant protein purification.
- Expert systems offer a valuable tool for optimizing large-scale protein separation processes.
- Integration of characterization, modeling, and expert systems enhances purification process development.