Kinetic calibration using dominant pre-equilibrium desorption for on-site and in vivo sampling by solid-phase...

Area of Science:

• Analytical Chemistry
• Environmental Science

Background:

• Matrix effects can complicate quantitative analysis in techniques like solid-phase microextraction.
• Traditional calibration methods often require specific internal standards, adding complexity and cost.

Purpose of the Study:

• To develop a new kinetic calibration method for solid-phase microextraction.
• To improve accuracy and robustness in quantitative analysis.
• To reduce sample preparation time and eliminate the need for specialized internal standards.

Main Methods:

• Developed a kinetic calibration based on dominant pre-equilibrium desorption.
• Utilized isotropism between absorption and desorption, verified experimentally.
• Employed concentration profiles for isotropism verification and linear time constant determination.
• Used target analytes as internal standards.

Main Results:

• Demonstrated isotropism in aqueous and semisolid matrices.
• Achieved accurate quantitative analysis through a linear approach for time constants.
• Successfully applied the method to polycyclic aromatic hydrocarbons and in vivo pesticide sampling.
• Eliminated the need for radioactive or deuterated internal standards.

Conclusions:

• The new kinetic calibration method offers a more convenient, robust, and accurate alternative.
• The method effectively compensates for matrix effects.
• Reduced sample preparation time makes it suitable for in vivo applications.