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Solid-phase extraction of midazolam and two of its metabolites from plasma for high-performance liquid
V Sautou1, J Chopineau, M P Terrisse
1Laboratoire de Pharmacologie et Pharmacie Clinique et Biotechniques, U.F.R. Pharmacie, Clermont-Ferrand, France.
Journal of Chromatography
|November 15, 1991
Abstract:
A rapid, sensitive and selective assay of midazolam and two of its metabolites in plasma, based on high-performance liquid chromatography, has been developed. The compounds are subjected to solid-phase extraction, using C18 cartridges (Bond-Elut). Recoveries are in excess of 90% for midazolam and its metabolites. The limit of quantitation of the assay is 50 ng/ml of plasma for each compound.