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Updated: Feb 14, 2026

DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes
Published on: March 31, 2019
Study of thiazole orange in aptamer-based dye-displacement assays
Renjun Pei1, Milan N Stojanovic
1Division of Experimental Therapeutics, Department of Medicine, Columbia University, New York, NY 10032, USA. rp2164@columbia.edu
Abstract:
We screened a series of RNA and DNA aptamers for their ability to serve in the dye displacement assays in which analytes compete with TO dye. We conclude that, while the performance of the TO dye displacement approach is not always predictable, it is still a simple and sensitive assay to detect binding between RNA aptamers and small molecules. In particular, we describe efficient assays for tobramycin and theophylline, with up to 90% displacement of TO observed, and we describe the first aptameric assay for cAMP.
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