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UV–Vis Spectrometers

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...

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Haematococcus pluvialis cell-mass sensing using ultraviolet fluorescence spectroscopy.

Abdolmajid Lababpour¹, Seong-Joo Hong, Choul-Gyun Lee

¹Institute of Industrial Biotechnology, Department of Biotechnology, Inha University, Incheon 402-751, Korea.

Journal of Microbiology and Biotechnology

|January 3, 2008

Summary

This study introduces a simple ultraviolet fluorescence spectroscopy method for measuring algal cell mass in Haematococcus pluvialis. The technique accurately quantifies biomass across different cell morphologies without pretreatment, offering a non-invasive online monitoring solution.

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Area of Science:

Biotechnology
Spectroscopy
Algal Biotechnology

Background:

Accurate measurement of algal biomass is crucial for optimizing cultivation and harvesting.
Existing methods for cell mass determination can be time-consuming or require sample pretreatment.
Haematococcus pluvialis is a valuable microalga for producing astaxanthin, necessitating efficient biomass monitoring.

Purpose of the Study:

To develop a simple, whole-cell-based sensing system for determining the cell mass of Haematococcus pluvialis.
To utilize ultraviolet fluorescence spectroscopy for non-invasive algal biomass quantification.
To establish a method applicable across various morphological states of H. pluvialis.

Main Methods:

Employing ultraviolet fluorescence spectroscopy with an excitation wavelength of 227 nm and monitoring emission at 368 nm.
Developing a calibration curve correlating fluorescence emission intensities with dry cell weight.
Validating the method's accuracy across different morphological forms (green, green-brown, brown-red, red) of H. pluvialis.

Main Results:

A strong correlation (R²=0.9938) was achieved between fluorescence emission intensity and H. pluvialis cell mass.
The method demonstrated high accuracy irrespective of the algal cell's morphological state.
Background interference from medium components was minimal and effectively subtracted.

Conclusions:

The proposed ultraviolet fluorescence spectroscopy method provides a reliable and non-invasive approach for H. pluvialis cell mass determination.
This technique eliminates the need for sample pretreatment, enabling direct online monitoring of algal biomass.
The system has significant potential for optimizing large-scale algal cultivation and biomass production.