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Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis
Published on: June 24, 2018
Calcium ion diffusion from mineral trioxide aggregate through simulated root resorption defects.
Hüseyin Ozgür Ozdemir1, Bahar Ozçelik, Bekir Karabucak
1Department of Endodontics, Faculty of Dentistry, Hacettepe University, Ankara, Turkey. dtozguroz@yahoo.com
Summary
Mineral trioxide aggregate (MTA) allows calcium ion (Ca+2) diffusion through dentinal tubules in teeth with root defects. This study measured Ca+2 release over 28 days, confirming MTA
Area of Science:
- Biomaterials Science
- Dental Research
- Endodontics
Background:
- Mineral trioxide aggregate (MTA) is widely used in endodontic procedures.
- Understanding ion diffusion through dentinal tubules is crucial for assessing MTA's sealing ability and biological effects.
- External root resorption defects present a challenge for MTA's sealing efficacy.
Purpose of the Study:
- To investigate the diffusion of calcium ions (Ca+2) through exposed dentinal tubules.
- To assess Ca+2 release from root defects following intracanal application of MTA.
Main Methods:
- Fifty-two single-rooted teeth were prepared and standardized defects created on root surfaces.
- Root canals were irrigated with sodium hypochlorite and ethylenediaminetetraacetic acid.
- MTA was placed in root canals of experimental group; control group had unfilled canals. Ca+2 release into saline was measured over 28 days.
Main Results:
- Diffusion of Ca+2 was observed through dentinal tubules in MTA-filled roots.
- A significant time-dependent increase in Ca+2 concentration was detected in the surrounding saline.
- Control teeth (unfilled canals) showed no significant Ca+2 release.
Conclusions:
- MTA facilitates the diffusion of calcium ions through dentinal tubules, even in the presence of root defects.
- The findings suggest MTA's potential to release ions that may influence the periapical environment.
- Further research is needed to correlate ion diffusion with clinical outcomes.
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