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Updated: Jul 8, 2026
Identifying Protein-protein Interaction Sites Using Peptide Arrays
Published on: November 18, 2014
[Method of antibody analysis based on visual protein array]
Zhe Yu1, Zhi-hong Liu, Ying-song Wu
1College of Life Science, Capital Normal University, Beijing 100037, China. yzhsd@163.com
Aim:
To establish a novel method for antibody analysis based on visual protein microarray.
Methods:
The antibodies spotted on the aldehyde-modified slides were incubated with corresponding antigens labeled by biotin, followed by silver enhancement detection method, and the chromogenic images were scanned by CCD camera.
Results:
The affinity and specificity of each antibody was assayed by this method and six antibodies were found to have higher specificity than the others. A detection limit of 0.4 microg/L was obtained for anti-globulinIIIC013, anti-albumin HPSIIB007, and anti-albumin HPSIIIB007.
Conclusion:
This method is simple, fast and visual. In addition, it needs less sample amount than traditional immunoassay and has potential to achieve high throughput.
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