Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Viral genome sequencing by random priming methods.

Appolinaire Djikeng1, Rebecca Halpin, Ryan Kuzmickas

  • 1Viral Genomics Group, J, Craig Venter Institute, Rockville, MD 20850, USA. adjikeng@jcvi.org

BMC Genomics
|January 9, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High quality genome assemblies of African cattle breeds using PacBio HiFi sequencing.

Scientific data·2026
Same author

Disulphide and sequence-encoded conformational priors guide nanobody structure prediction.

bioRxiv : the preprint server for biology·2026
Same author

Immunity to hemagglutinin and neuraminidase results in additive reductions in airborne transmission of influenza H1N1 virus in ferrets.

Science advances·2026
Same author

A chromosome-level genome assembly of the South African indigenous, Kolbroek pig, Sus scrofa domesticus.

Scientific data·2026
Same author

Erratum for Yuan et al., "Case-control and genomic epidemiology characterization of SARS-CoV-2 breakthrough infections during Delta-to-Omicron transition".

mBio·2026
Same author

BioStruct-Africa's scalable framework for AlphaFold-enabled research training and sustainable workforce development in Africa.

Communications biology·2026
Same journal

Tissue identity is the dominant determinant of cross-species transferability of a porcine developmental programme.

BMC genomics·2026
Same journal

Characterization of mitochondrial genomes from three medicinal species of rutaceae and comparative analysis within the family: insights into evolution.

BMC genomics·2026
Same journal

Comparative genomic analysis of Herbaspirillum strains associated with banana sheath rot reveals potential virulence factors.

BMC genomics·2026
Same journal

Transposable element disruption of a second thyroglobulin-like gene confers Vip3Aa resistance in Helicoverpa armigera.

BMC genomics·2026
Same journal

Molecular ontogeny of Bemisia tabaci life stages reveals miRNA-governed development and egg-stalk support for early embryogenesis.

BMC genomics·2026
Same journal

Genome-wide characterization of the accessible chromatin regions in chickpea.

BMC genomics·2026
See all related articles

A new method enhances viral genome sequencing for RNA and DNA viruses, aiding in the surveillance and prediction of emerging infectious diseases. This technique is crucial for understanding viral diversity and potential pandemic threats.

Area of Science:

  • Virology
  • Genomics
  • Infectious Diseases

Background:

  • Emerging health threats are frequently zoonotic, with RNA viruses being common causative agents.
  • Understanding global viral diversity is critical for pandemic surveillance and prediction.
  • Rapid and flexible methods for complete viral genome sequencing are needed.

Purpose of the Study:

  • To adapt and demonstrate the utility of the SISPA methodology for comprehensive viral genome sequencing.
  • To enable efficient sequencing of diverse RNA and DNA viruses.

Main Methods:

  • Adaptation of the SISPA methodology for both RNA and DNA virus genome sequencing.
  • Application of the method to various virus types and sample sources, including cell culture supernatant, allantoic fluid, and fecal matter.

Related Experiment Videos

Main Results:

  • Successful near-complete genome sequencing of viruses ranging from 3,000-15,000 kb.
  • Achieved a median depth of coverage of 14.33.
  • Generated full viral genome sequences in the presence of host contaminants.

Conclusions:

  • The adapted SISPA method is highly effective for whole genome assembly of viruses with limited or no prior sequence data.
  • The technique is valuable for characterizing divergent, uncharacterized, or mixed viral infections.
  • Facilitates enhanced understanding of viral diversity for improved public health preparedness.