Controlled cavitation-cell interaction: trans-membrane transport and viability studies.
Rory Dijkink1, Séverine Le Gac, Erwin Nijhuis
1Physics of Fluids, Faculty of Science and Technology, University of Twente, PO Box 217, 7500 AE Enschede, The Netherlands.
Physics in Medicine and Biology
|January 11, 2008
Summary
Cavitation bubble dynamics near surfaces can detach cells, with optimal detachment at a normalized distance of 0.65. Molecular uptake is maximized at distance 0, and remaining cells show minimal viability loss.
Area of Science:
- Fluid dynamics
- Cell biology
- Biomedical engineering
Background:
- Cavitation bubble dynamics near surfaces generate transient fluid flow.
- Cellular responses to cavitation, including molecular delivery and detachment, are crucial for applications.
Purpose of the Study:
- To investigate the effect of cavitation bubble stand-off distance on cell detachment and molecular uptake.
- To assess the impact of single cavitation events on cell viability.
Main Methods:
- Generating single cavitation bubbles using laser pulses at varying normalized stand-off distances (gamma = h/Rmax).
- Observing molecular delivery and cell detachment on a rigid surface with adherent cells.
- Assessing cell viability and metabolism using MTS assay.
Main Results:
- Maximum cell detachment occurred at a normalized stand-off distance of approximately 0.65.
- Maximum molecular uptake was observed as the stand-off distance approached 0.
- Minimal impact on the viability and metabolism of non-detached cells was detected.
Conclusions:
- Cavitation bubble dynamics can be precisely controlled to optimize cell detachment or molecular delivery by adjusting the stand-off distance.
- Single cavitation events have limited adverse effects on the viability of surrounding cells.
