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Updated: Jul 8, 2026

Mouse Model of Alloimmune-induced Vascular Rejection and Transplant Arteriosclerosis
07:05

Mouse Model of Alloimmune-induced Vascular Rejection and Transplant Arteriosclerosis

Published on: May 17, 2015

Endothelial cell activation contributes to the release of procoagulant microparticles during acute cardiac allograft

Olivier Morel1, Patrick Ohlmann, Eric Epailly

  • 1Fédération de Cardiologie des Hôpitaux Universitaires de Strasbourg, France. olivier.morel@chrustrasbourg.fr

The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation
|January 12, 2008
PubMed
Abstract

Insights

Procoagulant microparticles, especially E-selectin-bearing ones, can help diagnose acute allograft rejection after heart transplantation. These markers indicate endothelial cell activation and apoptosis, aiding in early detection.

Area of Science:

  • Cardiovascular Medicine
  • Transplantation Immunology
  • Biomarker Discovery

Background:

  • Circulating procoagulant microparticles are established indicators of vascular damage.
  • Microparticle phenotypes offer insights into the specific mechanisms of cell injury.
  • Acute allograft rejection following heart transplantation necessitates reliable diagnostic markers.

Purpose of the Study:

  • To evaluate the diagnostic utility of procoagulant microparticle levels and phenotypes in acute allograft rejection after heart transplantation.
  • To identify specific microparticle phenotypes associated with rejection episodes.

Main Methods:

  • Prospective investigation of microparticles in venous blood from 64 heart transplant recipients (23 with rejection, 41 without).
  • Analysis of plasma concentrations for cytokines, cytoadhesins, and platelet activation markers.
  • Univariate and multivariate statistical analyses to determine associations with allograft rejection.

Main Results:

  • Univariate analysis identified associations between allograft rejection and time elapsed post-transplant, cold ischemia time, and microparticles expressing E-selectin, Fas, and tissue factor.
  • Multivariate analysis revealed E-selectin-bearing microparticles as an independent predictor of allograft rejection (OR, 9.8; 95% CI, 1.36-71.4; p = 0.023).

Conclusions:

  • The profile of procoagulant microparticles in acute allograft rejection suggests underlying endothelial cell activation and Fas-mediated apoptosis.
  • E-selectin-bearing microparticles serve as an independent and informative marker for acute allograft rejection, even after accounting for graft characteristics.

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